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Achromosome-scalegenomesequenceofpitaya(Hylocereusundatus)providesnovelinsightsintothegenomeevolutionandregulationofbetalainbiosynthesis
第一作者
Jian-yeChen
第一单位
华南农业大学
通讯作者
Yong-huaQin
Abstract
背景回顾：Pitaya(Hylocereus)isthemosteconomicallyimportantfleshy-fruitedtreeoftheCactaceaefamilythatisgrownworldwide,andithasattractedsignificantattentionbecauseofitsbetalain-abundantfruits.
提出问题：Nonetheless,thelackofapitayareferencegenomesignificantlyhindersstudiesfocusedonitsevolution,aswellasthepotentialforgeneticimprovementofthiscrop.
主要研究：Herein,weemployedvarioussequencingapproaches,namely,PacBio-SMRT,IlluminaHiSeqpaired-end,10×Genomics,andHi-C(high-throughputchromosomeconformationcapture)toprovideachromosome-levelgenomicassemblyof‘GHB’pitaya(H.undatus,2n?=?2x?=?22chromosomes).
结果1-组装、注释：Thesizeoftheassembledpitayagenomewas1.41?Gb,withascaffoldN50of~.15?Mb.Intotal,27,protein-codinggenesand.31?MbofrepetitivesequencesintheH.undatusgenomewereannotated.
结果2-基因组演化之全基因组复制事件：PitayahasundergoneaWGT(whole-genometriplication),andarecentWGD(whole-genomeduplication)occurredafterthegammaevent,whichis
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